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Protein Purification System

GE AKTA Avant

GE AKTA Avant 25 is the intelligent optimization of design of experiment (DOE) experimental conditions. It is the best partner for modern purification process optimization with UNICORN6.0 software. It is a fully automatic intelligent preparative chromatography for method exploration and purification process optimization. The AKTA Avant 25 system has a maximum design flow rate of 25 ml/min for packing selection, method optimization and sample preparation in hundreds of milligrams. UNICORN version 6 control software improves purification efficiency. AKTA Avant provides a complete solution for fast, high quality protein separation while maintaining flexibility and process reliability.
Parameters

Control system: UNICORN 6.1 or later

Dimensions(length × width × height): 860 × 660 × 710 mm

Weight(with computer removed): 116 kg

Power supply: 100–240 V, 50–60 Hz

Power: 800VA

Enclosure protection rating: IP 21, IP 22 (wet surface)

System pump

Pump type: metering piston pump

Flow rate: 0.001 to 25 ml/min

Pressure range: 0 to 20 MPa (2900 lbs)

Viscosity range: 0.35 to 10 cP

Sample pump

Pump type: metering piston pump

Flow rate: 0.01 to 25 ml/min

Pressure range: 0 to 10 MPa (1450 lbs)

Viscosity range: 0.7 to 10 cP

Mixer and gradient formation

Mixing method: magnetic stirrer

Mixing volume: 0.6, 1.4, or 5 ml (ÄKTA avant 25)

Gradient flow range: binary 0.25 to 25 ml/min (ÄKTA avant 25), quaternary 0.5 to 25ml/min (ÄKTA avant 25)

Gradient formation accuracy: binary ± 0.5%, quaternary ± 1%

valve

Type:Rotary valve (except quaternary valve)

Quaternary valve type: four-port electromagnetic drive diaphragm valve

Optional valve: up to three additional valves

Number of entrances

Entrance A: 7 can be expanded to 14

Entrance B: 7 can be expanded to 14

Sample entry: 7 can be expanded to 14

Quaternary valve entrance: 4, can be expanded to 18

Distillate outlet valve:

Number of distillate exports: 10, expandable to 32

Sensor and monitor:

Pressure sensor position: system pump, sample pump, front of column, behind column:

Airsensor position: inlet A, inlet B, sample port

Optional position: before the sample inlet valve, after injection of the valve

Sensing principle: ultrasound



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