Number of mobile phases: 1-4
Flow range: 0.000 and 0.010-10.000ml/min, 0.001ml/min
Typical operating flow range: <0.075RSD or 0.02min SD between the larger
Flow accuracy: between ±1% or 10μl/min
Compression compensation: automatic, continuous
Delay volume: <650μl
Plunge rcleaning: built-in
Maximum operating pressure: 34.5MPa
Pressure pulse: <2.5% (1ml/min, degassed methanol, post pressure 1700psi)
Gradient range: 0-100%, 0.1% increment
Gradient accuracy: ±5%
Gradient accuracy: <0.15%RSD or 0.02min SD between the larger
Sample tray: 120 2ml sample bottles (standard)
Injection volume: 0.1-100μl (standard) / 0.1-2000μl optional sample cup
Injection times: 1-99 times / bottle
Injection accuracy: <0.5% (typically)
Memory effect: <0.1%
Injection accuracy: ±1μl (±2%)
Cooling degree: 4-40 °C, 1 °C increment (optional)
Waters Alliance 2695 separation module main unit (including quaternary pump, 120-bits auto sampler, online plunger cleaning unit, online vacuum degassing unit)
Waters Alliance series column oven
Waters Alliance HPLC is available in a variety of detectors
Sofeware: Empower3 personal Single System Add-On
Detector name | Scope of application | Characteristic | Detector |
---|---|---|---|
UV/VISWaters 2487 2489 | Detection of most common UV-absorbing organic substances and some inorganic substances | High sensitivity, low noise, wide linear range, and good selectivity | |
PDA/DADWaters 2996 2998 PDA | Most compounds with UV absorption | Scans full-wavelength chromatograms and spectra, chromatographic peak purity and qualitative properties of components | |
RIDWaters 2410 2414 | Compounds that do not absorb light in the ultraviolet range, such as polymers, sugars, organic acids, and triglycerides, are detected. | Universal detector with good sensitivity to detect compounds with low UV absorption | |
FLDWaters 474 2475 | Detection of fluorescent substances, suitable for trace analysis of polycyclic aromatic hydrocarbons and various fluorescent substances. Derivatization can also be carried out to detect compounds that are themselves non-fluorescent | High selectivity, only response to fluorescent substances; high sensitivity, minimum detection limit of 10-12ug/ml | |
ELSDWaters 2420 2424 | Detection of compounds that do not contain chromophores, such as: carbohydrates, lipids, polymers, underivatized fatty acids and amino acids, surfactants, drugs, etc | High sensitivity, no influence on mobile phase system | |
ELCDWaters 432 | Detection of halogen, sulfur, nitrogen compounds | High selectivity and high sensitivity |